SIRT1 -promoter activation after transfection of NFκB subunits. Reporter plasmids containing the SIRT1 promoter were cotransfected with expression vectors for different NFκB subunits in HEK-293 T cells. Luciferase reporter gene assay was done after 24 hours and protein concentration of cell extracts was used to normalize the results. To calculate relative values constitutive luciferase intensity (named control) was measured after transfection of the SIRT1 reporter plasmid without NFκB expression plasmids. n = 3. *P ≤0.05 versus control group.