Reproducibility and intraindividual variation over days in buccal cell DNA methylation of two asthma genes, interferon γ (IFNγ) and inducible nitric oxide synthase (iNOS)

Table 1 Primer sequences

Gene	Primer	Sequence
IFNγ	Forward	5'-AGAATGGTATAGGTGGGTATAATGG-3'
	Reverse	5'-Biotin-CAAAACAATATACTACACCTCCTCTA-3'
	Sequencing (CpG^-54)^a	5'-ATTATTTTATTTTAAAAAATTTGTG-3'
	Sequencing (CpG^-186)^a	5'-GGTGGGTATAATGGGTTTG-3'
iNOS	Forward	5'-TTAGGGTTAGGTAAAGGTATTTTTGTTT-3'
	Reverse	5'-Biotin-CAATTCTATAAAACCACCTAATAATCTTAA-3'
	Sequencing^b	5'-TAAAGGTATTTTTGTTTTAA-3'

^aSite based on previous studies of interferon (IFNγ) [15].
^bSite based on previous studies of inducible nitric oxide synthase (iNOS) [17, 23].

ISSN: 1868-7083