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Figure 4 | Clinical Epigenetics

Figure 4

From: Secondhand smoke in combination with ambient air pollution exposure is associated with increasedx CpG methylation and decreased expression of IFN-γ in T effector cells and Foxp3 in T regulatory cells in children

Figure 4

Methylation and expression of Foxp3 in T regulatory cells (Tregs) of all subjects. A. Comparison of overall methylation of examined cytosine nucleotide neighboring guanine (CpG) sites in Foxp3 in Tregs between Fresno subjects exposed to secondhand smoke (SHS) (FSHS) (n = 31), Fresno subjects not exposed to SHS (FnSHS) (n = 31), Stanford subjects exposed to SHS (SSHS) (n = 6), and Stanford subjects who were not exposed (SnSHS) (n = 34) groups. Methylation is reported as percent methylated CpG sites out of 13 total examined CpG sites; the cutoff for positive methylation at each site was set at 70% of all sequencing reactions. Error bars represent standard error of the mean (SEM). B. Comparison of expression of Foxp3 in Tregs between FSHS, FnSHS, SSHS, and SnSHS groups. Expression is reported as fold expression relative to the expression of the housekeeping gene beta-glucuronidase (GUS). Error bars represent SEM. C. Linear regression analysis of Foxp3 expression in Tregs vs. % methylation of Foxp3 from all SHS subjects. D. Linear regression analysis of Foxp3 expression in Tregs vs. % methylation of Foxp3 in all nSHS subjects. E. Linear regression analysis of Foxp3 expression in Tregs from all subjects vs. % methylation of Foxp3. *P < 0.05; n.s., not significant.

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Clinical Epigenetics

ISSN: 1868-7083

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