Fig. 1

Kinetics of γH2AX elimination in human cells after IR measured by flow cytometry. Human embryonic fibroblasts (HEF) and cells of human neuroblastoma cell line IMR32 were irradiated at the dose 6 Gy, after formaldehyde fixation and immunostaining with rabbit primary antibodies to γH2AX followed by FITC-conjugated anti-rabbit IgG the cells were subjected to flow cytometry analysis, and the median fluorescence intensity was measured for each time point after IR. Median fluorescence intensity values for each cell line were normalized to the median values obtained for unirradiated cells (K). The experiments were repeated three times and error bars represent one standard error