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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Combined inhibition of histone deacetylase and cytidine deaminase improves epigenetic potency of decitabine in colorectal adenocarcinomas

Fig. 3

Regulation of apoptosis and cell cycle-related genes and cell cycle analysis in colon cancer cells following decitabine, PBA and the combined drug treatment. A Depicted is a Venn diagram highlighting unique and common apoptosis-related gene regulations following decitabine, PBA and the combined drug treatment. Together, we identified 17 upregulated pro-apoptosis (A1) and 11 repressed anti-apoptosis regulated genes (A2). The histogram highlights the gene expression changes after different epi-drug treatments (A3). B Cell cycle-related gene regulations following daily decitabine, PBA and the combined drug treatment for 96 h. Drug treatment repressed 8 cell cycle genes with functions in G1 and G2/M phases (B1), respectively. The histogram shows the percentage repression relative to the DMSO controls (B2). C Cell cycle analysis of the 3 different colon cancer cell lines following decitabine, PBA and the combined daily treatment for 72 h. We performed FACS assays with the Dye Cycle Violet stain. The data are percentage cells in the different phases of the cell cycle (C1). The statistical analysis is based on an unpaired t-test of three independent experiments (C2). Drug treatment caused inhibition of cell cycle progression in different phases of the cell cycle

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