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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Differential effect of histone H3.3 depletion on retroviral repression in embryonic stem cells

Fig. 3

Altered chromatin conformation on proviral chromatin following H3.3 and Trim28 depletion. A ChIP-qPCR for Trim28 in WT and H3.3 depleted cells infected with PBSpro or B PBSgln virus. Data are the mean ± s.e.m. (n = 3) C anti-H3K9me3 ChIP-qPCR in WT and H3.3 depleted cells infected with PBSpro or D PBSgln virus. In A–D, Data are the mean ± s.e.m. (n = 3), P value was calculated using Bonferroni–Dunn method without assuming a consistent SD E RT-qPCR for Trim28 expression on WT and Trim28KD cells, normalized to UBC control gene (n = 6). P value was calculated using Kruskal–Wallis nonparametric test with Dunns' multiple comparison, **P < 0.01. F Percentage of GFP expressing cells following Trim28 depletion (n = 6). G ChIP-qPCR for H3.3-HA in WT and Trim28KD ESCs infected with PBSpro or PBSgln viral vectors (n = 2). In F, G, Data are the mean ± s.e.m., P value was calculated using Mann–Whitney U unpaired nonparametric test, *P < 0.05, **P < 0.01

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