Fig. 2

Selection of novel hits sparing for non-cancer cells. A We used data from knockout screens in > 300 traditional cancer cell lines listing for each gene how many of the cell lines (in percentage) that were dependent on the gene. The histogram shows the dependency distribution of all hits in our screen where hits with > 70% dependency are coloured in red. B The average rank of GU-pBT-7 and GU-pBT-19 (with technical duplicates for each cell line) vs dependent cell lines (according to Behan et al.) for all 154 hit genes in the screen. The dashed line indicates 70% dependency, which was used as a threshold for selecting 13 hits for individual validation (shown in purple). The hit genes above 70% dependency are coloured in red, and the remaining hits in blue. C Volcano plot of knockout screen results for GU-pBT-7 replicate 1 and D GU-pBT-19 replicate 2, of all genes in the screen where the 13 selected hits are labelled in purple. Hit genes above 70% dependency are coloured in red, remaining hit genes for each cell line is shown in green, and the remaining genes (non-hits) in the screen in grey