Fig. 7

In vivo and in vitro ARTs affect DNA methylation in regions implicated in different biological processes. Number of hypo- and hypermethylated differentially methylated regions (DMRs) in A in vivo and B in vitro groups relative to the control group. Number of DMCs merged within each hypo- and hypermethylated DMR for C in vivo and D in vitro groups. Genes annotated to DMRs containing the highest number of DMCs are identified. All significant biological processes from Gene Ontology analysis of all DMRs annotated to genic regions for E in vivo and F in vitro analyses. The number of observed genes associated with each term is indicated within bars. Enrichment is defined as the proportion of observed genes compared to the number annotated within the whole dataset and is indicated by bar colour. The dotted line represents the significance threshold: weighted Fisher’s p value < 0.01