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Table 1 Primer and Taqman probe sequences used to amplify bisulphite converted DNA in MethyLight analysis

From: SOCS1 methylation level is associated with prognosis in patients with acute-on-chronic hepatitis B liver failure

Gene

Primer and Taqman probe sequences (5′–3′)

SOCS1

Forward: GCGTCGAGTTCGTGGGTATTT

Reverse: CCGAAACCATCTTCACGCTAA

Probe: 6FAM-ACAATTCCGCTAACGACTATCGCGCA-TAMRA

Alu-C4

Forward: GGTTAGGTATAGTGGTTTATATTTGTAATTTTAGTA

Reverse: ATTAACTAAACTAATCTTAAACTCCTAACCTCA

Probe: 6FAM-CCTACCTTAACCTCCC-TAMRA

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Clinical Epigenetics

ISSN: 1868-7083

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