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Fig. 6 | Clinical Epigenetics

Fig. 6

From: Epigenetic priming improves salvage chemotherapy in diffuse large B-cell lymphoma via endogenous retrovirus-induced cGAS-STING activation

Fig. 6

cGAS-STING-mediated viral mimicry was dependent on reverse transcriptase activity. A–C Reverse transcriptase activity was measured after PBS, AZA, RTi, and AZA + RTi treatment on OCI-LY1, SU-DHL2, and SU-DHL8, respectively. D–F IC50 values were calculated using cell viability measured on day 5 after pretreatment with PBS, AZA, RTi, and the AZA + RTi combination on OCI-LY1, SU-DHL2, and SU-DHL8, respectively. G–I The expression of cGAS and STING and phosphorylation levels of IRF3 and IκBα were measured using western blotting after PBS, AZA, RTi, and AZA + RTi treatment on OCI-LY1, SU-DHL2, and SU-DHL8, respectively. J–L The expression of cGAS and STING and phosphorylation levels of IRF3 and IκBα were measured in the negative control and knockdown cell lines with and without 5-azacytidine treatment on SU-DHL8 cells. A and B *p < 0.05, **p < 0.01 and ***p < 0.001 (two-way ANOVA). AZA: 5-azacytidine, RTi: reverse transcriptase inhibitor (delavirdine mesylate)

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Clinical Epigenetics

ISSN: 1868-7083

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