Skip to main content
Fig. 6 | Clinical Epigenetics

Fig. 6

From: E3 ubiquitin ligase RNF180 prevents excessive PCDH10 methylation to suppress the proliferation and metastasis of gastric cancer cells by promoting ubiquitination of DNMT1

Fig. 6

RNF180 enhances DNMT1 ubiquitination and degradation. A Immunofluorescence assay showed RNF180 and DNMT1 were co-localized in MKN45 and HGC27 cells. Scale bar: 20 μm. B Coimmunoprecipitation (Co-IP) assay was performed to detect the interaction between RNF180 and DNMT1. C, D DNMT1 degradation was suppressed in MKN45-shRNF180 cells compared with that in MKN45-shcontrol cells, as indicated by CHX chase assay. E, F DNMT1 degradation was higher in HGC27-RNF180 cells compared with that in HGC27-vector cells, as indicated by CHX chase assay. G DNMT1 expression was detected by western blot in MKN45-shcontrol and MKN45-shRNF180 cells after being treated with DMSO or MG132. H DNMT1 expression was detected by western blot in HGC27-vector and HGC27-RNF180 cells after being treated with DMSO or MG132. I Western blot images showing DNMT1-associated ubiquitination (Ubi) in control and RNF180-knockdown MKN45 cells treated with MG132

Back to article page

Clinical Epigenetics

ISSN: 1868-7083

Contact us