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Fig. 5 | Clinical Epigenetics

Fig. 5

From: E3 ubiquitin ligase RNF180 prevents excessive PCDH10 methylation to suppress the proliferation and metastasis of gastric cancer cells by promoting ubiquitination of DNMT1

Fig. 5

RNF180 restored PCDH10 expression by inhibiting DNMT1. A, B Volcano plot of altered gene expression patterns in RNF180 overexpressed HGC27 cells identified from iTRAQ quantitative analysis. C The significantly dysregulated pathways among RNF180 overexpression and control groups in different KEGG categories. D Western blot showed downregulation or upregulation of RNF180 expression changed the DNMT1 expression in GC cells. E Heatmap of 20 dysregulated genes identified in HGC27-RNF180 and control cells using gene chip detection analysis. (F) Spearman’s correlation analysis of TCGA database indicated RNF180 expression was positively correlated with PCDH10 expression. G Western blot showed downregulation or upregulation of RNF180 expression changed the PCDH10 expression in GC cells. H PCDH10 methylation increased after RNF180 knockdown, as indicated by NGS methylation analysis. I Western blot analysis confirmed that DNMT1 overexpression rescued RNF180 overexpression-induced increase in PCDH10 expression. J Transwell assays revealed that DNMT1 upregulation partially countervailed the inhibitive effect of RNF180 overexpression on the invasion and migration of HGC27 cells. **p < 0.01, ***p < 0.001

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