Fig. 4From: E3 ubiquitin ligase RNF180 prevents excessive PCDH10 methylation to suppress the proliferation and metastasis of gastric cancer cells by promoting ubiquitination of DNMT1PCDH10 overexpression inhibits the migratory and invasive abilities of GC cells in vitro and in vivo. A Transwell assay was performed to determine the effects of PCDH10 overexpression on cellular invasion and migration. B Representative bioluminescence images of the different groups are shown at 8 weeks after tail vein injection (left). The radiance was collected and calculated (right). C Representative hematoxylin and eosin (H&E) staining images of lung tissue sections from different groups (left). The number of lung metastatic foci was calculated (right). Scale bars: 200 μm (main) and 50 μm (insert). D Heatmap showing differentially expressed genes in PCDH10-overexpressing HGC27 cells. E The interaction diagram of proteins involved cell growth, cytokine-mediated signaling pathway, and cell-substrate adhesion. F The significantly dysregulated pathways among PCDH10 overexpression and control groups in different KEGG categories. G RT-PCR analysis of target genes in indicated cells. *p < 0.05, ***p < 0.001, ****p < 0.0001, N.S. Not significantBack to article page