Skip to main content
Fig. 2 | Clinical Epigenetics

Fig. 2

From: E3 ubiquitin ligase RNF180 prevents excessive PCDH10 methylation to suppress the proliferation and metastasis of gastric cancer cells by promoting ubiquitination of DNMT1

Fig. 2

Decreased expression of PCDH10 is attributed to DNMT1-mediated promoter hypermethylation. A The global methylation level of PCDH10 in gastric cancer (GC) cells from the Broad Institute CCLE databases. B High PCDH10 methylation levels were associated with low mRNA levels from the Broad Institute CCLE database. C PCDH10 methylation level in GC tissues and adjacent nontumor tissues was confirmed by NGS methylation analysis. D High PCDH10 methylation levels were associated with low mRNA levels from the TCGA database. E, F, G Effect of 5-AZA treatment on mRNA, protein expression and methylation level of PCDH10. H, I Western blot and qPCR confirmed that DNMT1 knockdown increased the protein and mRNA expression of PCDH10. J, K NGS methylation analysis showed that DNMT1 knockdown suppressed the global methylation level of PCDH10 in AGS cells. **p < 0.01, ***p < 0.001

Back to article page

Clinical Epigenetics

ISSN: 1868-7083

Contact us