Fig. 3

Butyric acid can improve the metabolism and inflammatory response of granulosa cells in inflammatory state. A, B Edu staining was used to detect KGN cells proliferation treated with LPS and low-dose(L-BA) and high-dose (H-BA) butyric acid. Scale bar: 100um, *P < 0.05, **P < 0.01. C, D Caspase3 activity kit was used to detect apoptotic KGN cells treated with LPS and low-dose and high-dose butyric acid for 48 h. Relative caspase3 activity was calculated by average positive cell ratio (The results were shown as means ± SD) (n = 3). Scale bar: 100um, *P < 0.05, **P < 0.01. E, F Mitochondrial membrane potential was detected by JC-1 staining. Red fluorescence indicates high mitochondrial membrane potential, while green fluorescence indicates low mitochondrial membrane potential. The higher the red/green ratio, the fewer apoptotic cells. (The results were shown as means ± SD) (n = 3). Scale bar: 100um, *P < 0.05, **P < 0.01