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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinoma

Fig. 4

Upregulation of tumor suppressor genes by CRISPRa in HuH-7 HCC cells. A–F Reactivation of TSGs by CRISPRa was evaluated by qRT-PCR 48 h after transient transfection. Fold change in TSG mRNA expression from transfected cells with CRISPRa and TSG-targeting gRNAs, normal and immortalized hepatocytes was normalized to control transfections with empty vector (EV), and compared to CRISPRa with no gRNA (NO G) for statistical analysis. From left to right: A HHIP: ****P < 0.0001; B CPS1: ****P < 0.0001, ***P = 0.0005; C PZP: ****P < 0.0001; D TMEM106A: ****P < 0.0001, **P = 0.0063; E TTC36: ***P = 0.0001, ****P < 0.0001, ***P = 0.0003; F CDKN2A: ***P = 0.0010, *P = 0.0185, ****P < 0.0001. Data presented as means ± SEM (n = 3), and P values were determined by unpaired t-test

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