Fig. 5

The accuracy of peritoneal cfDNA for detection of PM and prediction of recurrence. A The cutoff value of MaxAF was identified at best threshold of 6.29% (AUC: 0.951) with 100% of sensitivity and 78.3% of specificity by ROC analyses. B In the training cohort, the sensitivity, specificity and accuracy of peritoneal cfDNA for PM detection were 100%, 78.3%, 87.5%, respectively. In the validation cohort, 83.3% of sensitivity, 100% of specificity and 90.9% of accuracy were shown. C Positive peritoneal cfDNA was associated with tumor recurrence (HR = 7.1 (95% CI: 1.18, 42.84), P = 0.013). D During follow-up, recurrence occurred in 3/5 (60%) of CRC patients with positive cfDNA (mean MaxAF 18.7%), while only 2/18 (11.1%) recurrences of lung metastasis were observed in CRC patients with negative cfDNA (P = 0.083)