Fig. 3

i-BET858 treatment leads to significant G2/M cell cycle arrest. A Flow cytometry cell cycle analyses of OVCAR-3 cells treated with different concentrations of i-BET151, i-BET858 and DMSO vehicle control for 24 and 48 h. Blue peaks represent cells in G0/G1 phase, whilst green peaks represent cells in G2/M phase. The area depicted as yellow represents cells in S phase. B–D Illustrative representations of the percentage of OVCAR-3, CAOV3 and SKOV3 cells present in different cell cycle phases following treatment. Particles containing less DNA than that of G0/G1 cells (< G0/G1) are related to apoptotic damage; due to low numbers this population was overlooked in graphs displaying 24 h treatment results. FlowJo™ was unable to fit peaks obtained at high concentrations of i-BET151 (2.5 µM) in CAOV3 cells; these datasets are included in Additional file 1: Fig. S2. E Table detailing specific percentages of cells detected per cell cycle phase following treatments