Fig. 4
Combined analyses of m6A modification and expression profiles of 143 differentially m6A-modified lncRNA transcripts in LUAD tissues. A The UpSet diagram was used to divide lncRNAs into six categories. B Spearman correlation analyses between the expression levels and methylation levels of 143 lncRNA transcripts in normal tissues (left), tumor tissues (middle), and all tissues (right). A point’s horizontal and vertical coordinates were the mean m6A level and mean expression level of a lncRNA transcript in six normal tissues (left), four tumor tissues (middle), and all ten tissues (right), respectively. C Validation of four lncRNAs’ expression levels in six pairs of clinical tissues; GAPDH served as the reference gene. *P < 0.05 and not significant (ns) P > 0.05. Paired t test. D Validation of four lncRNAs’ m6A levels in six pairs of clinical tissues by MeRIP-qPCR assays; Input was used as the reference. *P < 0.05, **P < 0.01 and ***P < 0.001. Paired t test