Fig. 5
From: Neuroglobin plays as tumor suppressor by disrupting the stability of GPR35 in colorectal cancer
NGB downregulates GPR35 by promoting its degradation. A, B The expression level of NGB and GPR35 explored by q-PCR and WB. C Protein interaction between NGB and GPR35 confirmed by IP. D IF: The effect of NGB on GPR35 expression in HCT116 and Caco2 cells. E The protein stability of GPR35 after CHX treatment was examined in NGB-OE or vector HCT116 cells. All experiments were performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001