Fig. 5

Comparative Tal2 gene expression analysis with RT-qPCR. A testis, B ovary, and C kidney between control F1 and SSCT offspring in F1 and F2/M and F2/P. The bar chart and statistical analyses with ANOVA followed by Tukey’s post hoc testing represent the RNA expression between groups of all generations, (n = 5 per organ, per offspring subgroup in the F1 and F2, RT-PCR performed in technical triplos). *Statistically significant differences observed between groups (p < 0.05). F2/M—F2 generated from the maternal line, F2/P—F2 generated from the paternal line