Fig. 2

miR-148/-152 family members target DNMTs 3′UTR. A Correlations among miR-148/-152 family members and DNMT expression levels in tumor tissues from 42 NSCLC cancer patients were analyzed using Spearman’s correlation coefficient. Magenta color indicates P < 0.05. B Expression patterns of the miR-148/-152 family were analyzed in tumor tissues relative to normal tissues of 18 lung adenocarcinoma patients selected from the TCGA dataset. C Hierarchical clustering analysis of differentially expressed miRNAs between A549 cells treated with metformin or not. Red and green represent upregulated and downregulated miRNAs, respectively. D–F A549 cells were treated with 0 mM and 10 mM metformin for 72 h. Expression levels of miR-148/-152 family members were measured by qRT‐PCR. Expression levels of miR-148a-3p (D), miR-148b-3p (E), and miR-152-3p (F) were normalized to that of U6 small nuclear RNA. Data shown are mean ± standard deviation (n = 3), and significant differences are indicated by *P < 0.05, **P < 0.01, ***P < 0.001