Fig. 2

Characterization of DMR1–DMR7. a DMRs between 3 t(8;21) and 25 non- t(8;21) acute myeloid leukemia (AML) samples. A total of 5393 hypermethylated regions [absolute methylation level of t(8;21)—non-(t8;21) AML > 0.2; hypermethylated DMR1] and 39 hypomethylated regions [absolute methylation level of t(8;21)—non-(t8;21) AML <  − 0.2; hypomethylated DMR1] were identified in the t(8;21) AML samples. b Genomic distribution of DMR1. c DMRs (DMR2) between the AE-positive SKNO-1 and AE-negative SKNO-1-siAE cell lines. A total of 10,819 hypermethylated DMR2 [absolute methylation level of SKNO-1—SKNO-1-siAE > 0.2] and 35,834 hypomethylated DMR2 [absolute methylation level of SKNO-1—SKNO-1-siAE < − 0.2] were identified. d Genomic distribution of DMR2. e Alignment of DMR2 with DMR1. DMR regions that both identified as DMR1 and DMR2 were recognized as DMR3. f Genomic distribution of DMR3. g–h Methylation profile alteration after DAC-based regimen treatment of g t(8;21) and h non-t(8;21) AML patients. i DMRs with the same change direction after treatment in the 2 t(8;21) AML patients were identified as DMR4, comprising 6875 hypermethylated DMR4 [absolute methylation level of de novo sample–CR sample > 0.2] and 3720 hypomethylated DMR4 [absolute methylation level of de novo sample–CR sample <  − 0.2]. j Genomic distribution of DMR4. k DMRs with the same change direction after treatment in the 3 non-t(8;21) AML patients were identified as DMR5, comprising 8596 hypermethylated DMR5 [absolute methylation level of de novo sample–CR sample > 0.2] and 117 hypomethylated DMR [absolute methylation level of de novo sample–CR sample <  − 0.2]. l Genomic distribution of DMR5. m Alignment of DMR5 with DMR4. DMR regions that only appeared in t(8;21) AML patients (DMR4) comprised DMR6. n Genomic distribution of DMR6. o Alignment of DMR3 with DMR6. Overlapped regions with the same change direction were identified as DMR7. p Genomic distribution of DMR7. q A cluster of downregulated genes in patients with the t(8;21) translocation compared with those without, according to TCGA-AML dataset. r Four genes were obtained through the intersection of the 72 hypermethylated genes derived from DMR7 with the 986 downregulated genes in t(8;21) AML. CR Complete response; DAC Decitabine