Fig. 3

EBS-seq is more effective for detecting 5hmCs than ACE-seq, a conventional method for high-resolution 5hmC detection. A Bar chart showing the number of 5hmC signals detected by ACE-seq and three replicates of EBS-seq in three cytosine contexts. B Bar chart showing the proportion of 5hmC signals detected by ACE-seq and three replicates of EBS-seq in each cytosine context across the whole genome. C, D EBS-seq was able to identify a larger number of CpG sites when compared to ACE-seq. EBS-seq detected more (C) CpG sites overall, and (D) high-confidence CpG sites that have more than three 5hmC signals