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Fig. 1 | Clinical Epigenetics

Fig. 1

From: EBS-seq: enrichment-based method for accurate analysis of 5-hydroxymethylcytosine at single-base resolution

Fig. 1

Enrichment-based single-base resolution 5hmC sequencing (EBS-seq) method. A Schematic of the EBS-seq method. B Illustration of selective 5hmC labeling and deamination of unmodified cytosine and 5mC. C Deamination efficiency of cytosine, 5-methylcytosine, and 5-hydroxymethylcytosine in spike-in DNA. D Heatmap showing correlations between replicates. Heatmap was created using RPKM-normalized read counts within sliding 10 kb windows. Correlation coefficients were calculated using Pearson correlation

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