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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Phospholipase C delta 1 inhibits WNT/β‐catenin and EGFR-FAK-ERK signaling and is disrupted by promoter CpG methylation in renal cell carcinoma

Fig. 5

Cell cycle and apoptosis analyses of PLCD1. a Effects of PLCD1 on the cell cycle distribution. The bar graphs described the percentage of cells in G0/G1, S and G2/M phases. b Cell apoptosis was analyzed by propidium iodide (PI) and Annexin V-FITC staining and assessed using flow cytometry. The percentage of early apoptotic cells in each group was shown in the quantification histogram. c Immunoblotting analysis of apoptosis-related markers, cleaved-caspase 3 (cleaved-Casp3), caspase 3, cleaved-PARP, PARP, Bax and Bcl-2. Relative protein-level ratio quantified by ImageJ was shown as histogram

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Clinical Epigenetics

ISSN: 1868-7083

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