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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Phospholipase C delta 1 inhibits WNT/β‐catenin and EGFR-FAK-ERK signaling and is disrupted by promoter CpG methylation in renal cell carcinoma

Fig. 4

PLCD1 overexpression attenuated EMT in A498 and HH244 cells. Immunofluorescence analysis of vimentin a and E-cadherin b in RCC cells transfected with PLCD1 plasmid or vector only. Cell nucleus were visualized by DAPI counterstaining. c Positive association between PLCD1 and CDH1 expression level analyzed by TIMER2.0 website. d Western blot analysis of E-cadherin, vimentin, Twist, MMP7 and PLCD1 with GAPDH as loading control. Graphs represent quantification of bands intensity with fold change compared with controls. e Morphological changes of A498 cells overexpressing PLCD1 relative to cells transfected with vector only. Scale bar: 45um. f Subcellular localization of PLCD1 and phalloidin staining. U-2 OS is a human osteosarcoma cell. The images were picked from Human Protein Atlas. Scale bar: 20um. CDH1: Cadherin1

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Clinical Epigenetics

ISSN: 1868-7083

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