Fig. 3
Effect of PLCD1 expression on cell viability, migration and invasion in A498 and HH244 cells. a Western blot analysis confirming the ectopic PLCD1 expression in A498 and HH244 cells by transfecting with the pcDNA3.1-PLCD1 plasmid. b Cell viability was evaluated using Cell Counting Kit-8 (CCK-8) assay, and absorbance at 450 nm was measured to reveal the proliferation rates at each time point. c Representative colony formation assays performing in vector- or PLCD1-expressing HH244 and A498 cells. The histogram was quantitative analysis of colony-formed numbers in each group. d Representative results of transwell migration and invasion e assays in HH244 and A498 cells. Migratory or invasive cells on the underside membrane of the chambers were fixed and stained with crystal violet and then counted under a light microscope. f, g Wound-healing assay in RCC cell lines, HH244 f and A498 g cells, transfected with vector alone or with pcDNA3.1-PLCD1 plasmid. Images were taken at 0, 12, 24 and 48 h by microscopy. The percentage of open wound over time was analyzed with line charts. The symbols *, ** and *** in these figures indicated p < 0.05, p < 0.01 and p < 0.001, respectively