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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Phospholipase C delta 1 inhibits WNT/β‐catenin and EGFR-FAK-ERK signaling and is disrupted by promoter CpG methylation in renal cell carcinoma

Fig. 1

PLCD1 expression and promoter CpG methylation analysis. a PLCD1 expression profile across human adult normal tissues examined by RT-PCR assay. b Expression of PLCD1 in RCC cell lines and human normal embryonic kidney cells, GAPDH was used as a reference gene. MSP was used to assess PLCD1 promoter methylation in RCC cell lines and immortalized epithelial cells. M: methylated, U: unmethylated. c CpG island at PLCD1 promoter identified by Database of CpG islands and Analytical Tool (DBCAT). Bent-tailed arrow denotes transcriptional start site. d BGS result confirmed PLCD1 promoter methylation in RHEK-1, A498, Caki-2, RCC98, ACHN and HH244 cells. Full circle represents methylated site, and open circle is unmethylated site. e PLCD1 restoration by demethylation treatment using 5-Aza alone or 5-Aza combined with TSA (A + T) in tumor cell lines, accompanied by promoter demethylation. RCC Renal cell carcinoma

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Clinical Epigenetics

ISSN: 1868-7083

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