Table 4 Methylation panels investigated in plasma or serum from OC and controls for early diagnosis of OC, including information on methods, methylation frequency, and performance

APC,APKinase, BRCA1, p14ARF, p16INK4a, RASSF1A

de Caceres [18]

MSP

50/10^b/20^h (T)

41/50

0/10^b

0/20^h

82.0 (68.6–91.4)

100* (88.4–100)

SOX, PAX1, LMX1A, SFRP1, SFRP2, SFRP5

Su [19]

MSP

26/20^b (T)

19/26*

9/20*

73.1 (52.2–88.4)

55.0 (31.5–76.9)

SOX, PAX1, SFRP1

Su [19]

MSP

26/20^b (T)

19/26*

5/20*

73.1 (52.2–88.4)

75.0 (50.9–91.3)

BRCA1, HIC1, PAX5, PGR, THBS1

Melnikov [20]

MSRE PCR/microarray

33/33^h (T)

28/33*

13/33*

85.1 (68.1–94.9)

61.1 (42.1–77.1)

CALCA, EP300, RASSF1A

Liggett [24]

MSRE PCR/microarray

30/30^h (T)

27/30*

4/30*

90.0 (73.5–97.9)

86.7 (69.3–96.2)

PGR, RASSF1A

Liggett [24]

MSRE PCR/microarray

30/30^b (T)

22/30*

6/30*

73.3 (54.1–87.7)

80.0 (61.4–92.3)

APC, CDH1, OPCML, RASSF1A, RUNX3, SFRP5, TFPI2

Zhang [27]

MSP, Nested multiplex

87/53^b (T)

39/29^b (V)

78/87

36/39

5/53

5/29

89.7 (81.3–95.2)

92.3 (79.1–98.4)

90.6 (79.3–96.9)

82.8 (64.2–94.2)

OPCML, RUNX3, TFPI2

Wang [30]

MSP, Nested multiplex

71/43^b/80^h (T)

64/71

11/123*

90.1 (80.7–95.9)

91.1 (84.6–95.5)

COL23A1, C2CD4D, WNT6

Widschwendter [34]

Targeted NGS

29/119^b/21^h (T)

48/154^b/41^h (V)

12/29

28/48

13/140

16/195

41.4 (23.5–61.1)

58.3 (43.2–72.4)

90.7 (84.6–95.0)

91.8 (87.0–95.2)

CDH1, PAX1, PTEN, RASSF1

Dvorská [36]

Pyrosequencing

33//9^h (T)

30/33*

4/9*

91.0 (75.7–98.1)

56.0 (21.2–86.3)

Panel of 103,456 distinct regions and 1,116,720 CpGs

Liu [11]

Targeted NGS

27/1521^h (T)

12/610^h (V)

26/27

12/12

3/1521*

4/610*

96.0 (81.0–99.9)

100 (73.5–100)

99.8 (99.4–100)

99.3 (98.3–99.8)

HIC1, HOXA9

Singh [39]

Real-time qMSP

45/25^h (T)

40/45

0/25

88.9 (76.0–96.3)

100 (86.3–100)

HIC1, HOXA9

Singh [42]

Real-time qMSP, Multiplex

45/25^{h ■} (T)

40/45

0/25

88.9 (76.0–96.3)

100 (86.3–100)

HOXA9, SOX1

Singh [42]

Real-time qMSP, Multiplex

45/25^{h ■} (T)

30/45

1/25

66.7 (51.1–80.0)

96.0 (79.7–99.9)

HIC1, SOX1

Singh [42]

Real-time qMSP, Multiplex

45/25^{h ■} (T)

36/45

1/25

80.0 (65.4–90.4)

96.0 (79.7–99.9)

AGRN, BCAT1, CAPN2, CDO1, CELF2, FAIM2, GPRIN1, GYPC, RIPPLY3, SRC, SIM2

Marinelli [44]

TELQAS

91/91^h (T)

72/91*

4/91*

79.0 (69.3–86.9)

96.0 (89.1–98.8)