Fig. 9
Identification of m7G methylation-related hub genes in HF. A Sample clustering was conducted based on the expression data of all HF samples. The top 25% variation genes were used for WGCNA, and outlier samples were excluded. The red line indicates the cutoff threshold (60). B Scale-free topology index analysis and mean connectivity of soft threshold power from 1 to 20. The red line indicates the scale-free R2 (0.879). C Clustering dendrogram of module eigengenes. The red line indicates the cut height (0.20). D Gene dendrogram obtained by average linkage hierarchical clustering. The genes were clustered into different modules through hierarchical clustering and merged when the correlation of the modules is > 0.8. E Heatmap of the correlation between module eigengenes and HF gene subgroups. F Correlation between module membership (X-axis) and gene significance (Y-axis) of genes from the blue module. G Protein–protein interaction (PPI) network of genes from the blue module. The central nodes in PPI network are marked in red, yellow, and orange