Fig. 4
Development and validation of the m7G regulator diagnostic signature for HF. A Forest plot of the multivariate logistic regression analysis to investigated the relationship between the five m7G regulator diagnostic markers and HF. B Nomogram of the five-gene m7G regulator diagnostic signature for HF probability. C receiver operating characteristic (ROC) curve of the five-gene m7G regulator diagnostic signature in the merged dataset. D The GiViTi calibration belts of the five-gene m7G regulator diagnostic signature in the merged dataset. E The expression profiles of the five m7G regulators diagnostic markers in the external validation dataset GSE116250. **p < 0.01, and ***p < 0.001 vs. the NFD group. F The expression profiles of five m7G regulators diagnostic markers in the external validation dataset GSE46224. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. the NFD group. G Validation of the 5 m7G regulators diagnostic markers expression (CYFIP1, DCP2, LARP1, NUDT4, and NUDT16) by quantitative real-time reverse-transcription PCR (qRT-PCR) using human heart tissues from HF patients and NFDs. Data are presented with mean ± standard deviation (SD), n = 8. **p < 0.01, and ***p < 0.001 vs. the NFD group. NS, no significance. H Validation of the 5 m7G regulators diagnostic markers expression by qRT-PCR using plasma samples from HF patients and NFDs. Data are presented with mean ± SD, n = 8. NS**p < 0.01, ***p < 0.001, and ****p < 0.0001 vs. the NFD group. NS, no significance