Fig. 4

Integrated analysis of AMA-related changes in both DNA methylome and transcriptome. A Venn diagram displays the relationship between AMA-related DEGs and the nearest genes of AMA-related DMRs. B Heatmaps present the scaled gene expression level (left) of AMA-related DEGs and scaled DNA methylation level (right) of DMRs in each sample. The gene symbols of DEGs or the nearest genes of AMA-related DMRs identified in (A) are shown to the right of the heatmap, respectively. C Scatter diagram shows the correlation between the gene expression level and the DNA methylation level of the nearby DMRs of six target AMA-related DEGs (AK5, GNE, KBTBD8, NUMBL, BIN1, KBTBD8, and LFNG). The straight line refers to the fitted linear regression line. The correlation coefficient and P value were calculated using Spearman-based correlation tests, and the shaded areas represent 95% confidence intervals around each slope