Fig. 2
From: Liver saturated fat content associates with hepatic DNA methylation in obese individuals
Genomic location enrichment for CpGs associated with SFA and/or NASH in human liver. Percentage of all CpGs located in relation to (A) nearest gene regions and (B) CpG island having important implications for the regulation of gene expression for only-NASH, NASH-SFA, and only-SFA groups, with percentage of all 450 K CpGs as control. CpG islands were defined as DNA sequences (as 500 base windows; excluding most repetitive Alu-elements) with a GC base composition greater than 50% and a CpG observed ratio [31] of more than 0.6. TSS1500-region within 1500 base pair upstream of a transcription start site; TSS200-region within 200 base pair upstream of a TSS; UTR-Untranslated region; N_Shore-2 kb regions upstream of CpG island; S_Shore-2 kb regions downstream of CpG island; N_Shelf-2 kb regions upstream of CpG island shore; S_Shelf-2 kb regions downstream of CpG island shore; and Open Sea-regions > 4 kb from CpG islands. *Indicates that the proportion of CpGs in only-NASH (n = 20,571), NASH-SFA (n = 797), and only-SFA (n = 2372) was significantly (p value < 0.01) different compared to all sites covered on the 450 K BeadChip (All 450 K), (χ2 test of independence with 1° of freedom). Each bar represents % of sites