Skip to main content
Fig. 2 | Clinical Epigenetics

Fig. 2

From: Azithromycin attenuates wheezing after pulmonary inflammation through inhibiting histone H3K27me3 hypermethylation mediated by EZH2

Fig. 2

Expression levels of H3K27me3 and EZH2 in BALF of patients with wheezing after pulmonary inflammation. A Histone H3K27me3 in the BALF was detected by immunofluorescence staining. Red (H3K27me3), blue (DAPI). The scale bars indicate 20 μm. B Average optical density of H3K27me3 immunofluorescence in the three groups (n = 3). C The expression level of histone H3K27me3 in the BALF was detected by Western blotting (n = 10). D The expression level of EZH2 in the sediment of BALF was detected by immunofluorescence staining. Red (EZH2), blue (DAPI). The scale bars indicate 20 μm. E Average optical density of EZH2 immunofluorescence in the three groups (n = 3). F The mRNA expression of EZH2 in the BALF was detected by RT-qPCR (n = 10). G The expression level of EZH2 in the BALF was detected by Western blotting (n = 10). *P < 0.05 versus the Control group and Non-wheeze group

Back to article page