Fig. 3

Rescue of ZSCAN18 expression inhibits the proliferation of GC cells via triggering autophagy. A Overexpression of ZSCAN18 in AGS and NCI-N87 cells was evidenced by qRT-PCR (left) and western blot (right). B Growth curves representing cell viability were inhibited by ZSCAN18 in both AGS and NCI-N87 cells. C Overexpression of ZSCAN18 suppressed colony formation in both AGS and NCI-N87 cells. D Tumor growth was inhibited in nude mice subcutaneously inoculated NCI-N87 cells with ZSCAN18 overexpression compared with control ones. E The KEGG enrichment plots of DEGs were generated from mRNA sequencing analysis of ZSCAN18-overexpressed and control NCI-N87 cells. F The expression of typical autophagy-related proteins was detected by western blot in ZSCAN18-overexpressed AGS and ZSCAN18-overexpressed NCI-N87 cells. G Immunofluorescent micrographs showed autophagosomes in both AGS and NCI-N87 cells. H The expression of typical autophagy-related proteins was detected by western blot in ZSCAN18-overexpressed AGS and ZSCAN18-overexpressed NCI-N87 cells pretreated with CQ/RAP. I The cell viability was detected by ATP in ZSCAN18-overexpressed AGS and ZSCAN18-overexpressed NCI-N87 cells after co-incubated with CQ/RAP. Each experiment was performed in triplicate and repeated at least three times. Data were represented as mean ± SD. **p < 0.01, ***p < 0.001, ****p < 0.0001