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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Inactivation of ZSCAN18 by promoter hypermethylation drives the proliferation via attenuating TP53INP2-mediated autophagy in gastric cancer cells

Fig. 2

Demethylation upregulated ZSCAN18 expression in GC cell lines. A ZSCAN18 mRNA expression was reduced in all GC cell lines. GES-1, normal gastric epithelial cell line. B The methylation level of each GC cell line was significantly higher than that of GES-1. C Correlation between mRNA expression and methylation level of ZSCAN18 in GC cell lines. D The mRNA expression of ZSCAN18 in AGS and NCI-N87 cells was restored after treatment with demethylation agent 5-Aza-2′-deoxycytidine. E Demethylation of ZSCAN18 was observed in AGS cells after treatment with demethylation agent 5-Aza-2′-deoxycytidine as examined by MassARRAY analysis. F Methylation level of ZSCAN18 was detected in a variety of CpG islands by MassARRAY analysis. Each experiment was performed in triplicate and repeated at least three times. Data were represented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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