Fig. 2

PedBE calculated DNAm age versus chronological age at 2-year follow-up—univariate plot. The chronological age at 2-year follow-up (x-axis) is plotted versus the PedBE estimated DNAm age (y-axis) for former PICU-patients and healthy control children. The red line indicates the linear regression for the former PICU-patients, the green line for the healthy control children. The grey zones indicate the 95% confidence interval. An increasing difference between former PICU-patients and healthy controls with age in the DNAm age-deviation was present. Separation of the curves started around the age of ± 8 years at 2-year follow-up (equivalent to ± 6 years at PICU admission). The inserted panel indicates the chronological age at 2-year follow-up (x-axis) versus the PedBE-estimated DNAm age (y-axis) for former early-PN versus late-PN PICU-patients. The blue line indicates the linear regression for the former early-PN PICU-patients, the orange line for former late-PN patients. The grey zones indicate the 95% confidence interval. No difference in DNAm age-deviation was present between former early-PN and late-PN PICU-patients. PICU: paediatric intensive care unit, PN: parenteral nutrition