Fig. 1

Global DNA methylation in mouse blastocysts from natural ovulation and superovulation groups. a In vivo matured MII oocytes were collected from adult mice (natural ovulation) and superovulated adult or prepubertal mice (superovulation). Superovulation was induced with an intraperitoneal injection of 2.5 IU (prepubertal) or 5 IU (adult) of eCG followed 48 h later by another intraperitoneal injection of the same dose of hCG. All MII oocytes underwent IVF. On Day 5 hatched blastocysts were collected. Right panels: Representative images of blastocysts on day 5 of embryo culture selected for PBAT. b Representative genome browser region showing the DNA methylation levels in naturally ovulated oocytes and NO, SOa and SOp blastocysts; the profile for each represents the merged PBAT data per group. Error bars indicate standard deviation. c Beanplots indicating whole-genome DNA methylation levels in individual blastocysts. The beanplots depict the density distribution of % CpG methylation of evaluated 100-CpG tiles common to all datasets (n = 206,059); within each beanplot, boxplot shows median value and 25–75th percentiles and whiskers show the lowest and highest observation. d Principal component analysis (PCA) of DNA methylation profiles for all 12 individual blastocysts. eCG, equine chorionic gonadotropin; hCG, human chorionic gonadotropin; IVF, in vitro fertilization; IVC, in vitro culture; NO, natural ovulation; SOa, superovulation adult; SOp, superovulation prepubertal. WGBS, whole-genome bisulfite-sequencing