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Table 2 Methylation difference of HTRA1 between 190 stroke cases and 190 controls in the case–control study

From: HTRA1 methylation in peripheral blood as a potential marker for the preclinical detection of stroke: a case–control study and a prospective nested case–control study

CpG sites

Controls

Stroke cases

Crude OR (95% CI)

P value

OR (95% CI)

P value

OR (95% CI)*

P value*

Median (IQR)

Median (IQR)

Per + 10% methylation

Per + 10% methylation

Per + 10% methylation

HTRA1_A_CpG_1

0.09 (0.05–0.12)

0.10 (0.07–0.13)

1.27 (0.92–1.75)

0.146

1.27 (0.92–1.76)

0.140

1.22 (0.85–1.77)

0.284

HTRA1_A_CpG_2

0.65 (0.56–0.73)

0.64 (0.58–0.74)

1.01 (0.92–1.12)

0.803

1.01 (0.92–1.12)

0.821

0.99 (0.88–1.11)

0.860

HTRA1_A_CpG_3

0.29 (0.22–0.35)

0.29 (0.24–0.35)

0.96 (0.78–1.17)

0.662

0.95 (0.78–1.16)

0.636

0.92 (0.73–1.15)

0.466

HTRA1_A_CpG_4

0.12 (0.09–0.15)

0.13 (0.11–0.15)

1.48 (0.96–2.30)

0.078

1.47 (0.95–2.28)

0.087

1.29 (0.79–2.11)

0.310

HTRA1_A_CpG_5

0.37 (0.31–0.45)

0.38 (0.30–0.45)

0.99 (0.86–1.14)

0.891

0.99 (0.86–1.14)

0.903

0.99 (0.85–1.16)

0.913

HTRA1_A_CpG_6

0.12 (0.10–0.15)

0.10 (0.06–0.13)

0.50 (0.32–0.78)

0.002

0.51 (0.33–0.79)

0.003

0.54 (0.34–0.86)

0.009

HTRA1_A_CpG_7

0.36 (0.28–0.45)

0.37 (0.30–0.45)

1.08 (0.92–1.27)

0.343

1.07 (0.92–1.26)

0.378

1.09 (0.91–1.30)

0.369

HTRA1_B_CpG_1

0.16 (0.09–0.41)

0.18 (0.11–0.44)

1.04 (0.95–1.13)

0.384

1.04 (0.96–1.14)

0.333

1.05 (0.95–1.16)

0.308

HTRA1_B_CpG_2

0.09 (0.05–0.12)

0.10 (0.07–0.14)

1.45 (1.02–2.06)

0.039

1.45 (1.02–2.06)

0.040

1.25 (0.86–1.82)

0.243

HTRA1_B_CpG_3.4

0.28 (0.24–0.33)

0.29 (0.25–0.34)

1.18 (0.90–1.55)

0.236

1.19 (0.90–1.57)

0.221

1.11 (0.81–1.52)

0.513

HTRA1_B_CpG_5

0.26 (0.20–0.34)

0.28 (0.22–0.33)

1.09 (0.90–1.33)

0.378

1.10 (0.90–1.34)

0.367

1.06 (0.85–1.32)

0.612

HTRA1_B_CpG_7

0.47 (0.36–0.54)

0.47 (0.37–0.54)

1.06 (0.92–1.22)

0.456

1.06 (0.92–1.22)

0.448

1.01 (0.85–1.19)

0.957

HTRA1_B_CpG_8.9

0.28 (0.24–0.33)

0.29 (0.25–0.34)

1.18 (0.90–1.55)

0.236

1.19 (0.90–1.57)

0.221

1.11 (0.81–1.52)

0.513

HTRA1_B_CpG_10

0.61 (0.53–0.71)

0.63 (0.55–0.70)

1.06 (0.92–1.23)

0.445

1.06 (0.92–1.23)

0.423

1.03 (0.87–1.22)

0.722

HTRA1_B_CpG_11.12

0.72 (0.65–0.80)

0.73 (0.66–0.80)

1.09 (0.92–1.30)

0.315

1.10 (0.92–1.31)

0.300

1.07 (0.88–1.31)

0.504

HTRA1_B_CpG_13.14.15

0.49 (0.41–0.57)

0.51 (0.45–0.57)

1.15 (0.97–1.36)

0.115

1.15 (0.97–1.36)

0.109

1.12 (0.92–1.36)

0.261

HTRA1_B_CpG_16

0.53 (0.45–0.63)

0.53 (0.45–0.68)

1.03 (0.91–1.17)

0.636

1.03 (0.91–1.17)

0.658

0.99 (0.86–1.15)

0.911

  1. Bold values indicated P < 0.05
  2. Logistic regression, adjusted for age and sex;
  3. *Logistic regression, adjusted for age, sex, smoking, drinking, hypertension, diabetes, TC, TG, HDL-C and LDL-C