Fig. 7

COL12A1 regulates the EMT program of iCCA cells. A, B Scatter plot showing the relationship between vimentin (VIM)/COL12A1 mRNA expression and the EMT-gene set enrichment score in three iCCA transcriptomic datasets (including E-MTAB-6389, TCGA and OEP001105). p value is based on Pearson correlation test. C The EMT-associated proteins in whole cell lysate of HuCCT1 and RBE cells with or without COL12A1 knockout were evaluated by immunoblotting. Bar plots showing the relative expression level of EMT-associated proteins in the two groups. Experiments were in triplicates. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, based on two-tailed unpaired Student's t test. iCCA intrahepatic cholangiocarcinoma, EMT epithelial–mesenchymal transition