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Table 1 Methylation levels at 11p15 H19/IGF2:IG-DMR of 10 iPSC clones (C6–C15). C6–C12 were reprogrammed from skin fibroblasts and C13–C15 from peripheral blood mononuclear cells (PBMCs). The reprogramming of somatic cells into iPSCs was carried out using mRNA transfection (C6–C11) or an episomal vector (C12–C15). iPSCs have been cultivated in classical culture conditions

From: Maintenance of methylation profile in imprinting control regions in human induced pluripotent stem cells

Clone

Somatic cells of origin

Technic of reprogramming

Culture conditions

11p15 H19/IGF2:IG-DMR, MI (%)

C6

Skin fibroblast

mRNA transfection

KSR feeder-free medium

Normoxia

96

C7

Skin fibroblast

mRNA transfection

80

C8

Skin fibroblast

mRNA transfection

70

C9

Skin fibroblast

mRNA transfection

68

C10

Skin fibroblast

mRNA transfection

89

C11

Skin fibroblast

mRNA transfection

66

C12

Skin fibroblast

Episomal vector

mTeSR1 feeder-free medium

Normoxia

67

C13

PBMC

Episomal vector

64

C14

PBMC

Episomal vector

67

C15

PBMC

Episomal vector

68