Fig. 5

A Methylation levels of 16 ERC-derived iPSCs clones at passage 4 cultivated in mTeSR medium in normoxia (classical conditions) and nine PBMC-derived iPSC clones at passage 5 (nine clones of iPSCs from one control) cultured with epiPS™ medium in normoxia or hypoxia at 11p15 H19/IGF2:IG-DMR (ICR1) (a) and 14q32 DLK1/MEG3:IG-DMR (b). The hatched part represents the average normal MI area for each locus. Analysis using Wilcoxon test (comparison of epiPS™ in Normoxia and epiPS™ in hypoxia) and Mann–Whitney test (comparison classical conditions with epiPS™ in Normoxia and epiPS™ in Hypoxia). B Morphology of iPSC cultured with epiPS™ medium in hypoxia or normoxia conditions. The iPSC clone cultivated with epiPS™ medium in normoxia displays a critical spontaneous differentiation unlike the same iPSC clone cultivated with epiPS™ medium in hypoxia. Same clone at passage 5, enlargement × 10