Fig. 6
Genes associated to estrogenic or non-estrogenic transposable regulatory sequences in sperm fraction sorted based on YOPRO− uptake. The observed number of genes associated to the DMR in the sorted sperm fractions of infertile patients and healthy semen donors and their relationship with ERE in the regulatory segments of those genes were compared with the expected numbers and differences between both were evaluated using chi-squared (X2)-tests (A). Contrasting differences between the observed and the expected numbers of genes associated to hypomethylated non-estrogenic Alu sequences were detected in the YOPRO− sorted sperm fractions. In addition, more genes were detected associated with hypermethylated short transposable regulatory sequences in the YOPRO− positive sperm fractions (p = 0.005). Based on the X2-values, heat maps were constructed to visualize differences between observed and expected numbers of associated genes in the various comparisons (B)