Fig. 5

Genes associated to estrogenic or non-estrogenic transposable regulatory sequences in sperm fraction sorted based on CMA3− uptake. The observed number of genes associated to the DMR in the sorted sperm fractions of infertile patients and healthy semen donors and their relationship with ERE in the regulatory segments of those genes were compared with the expected numbers and differences between both were evaluated using chi-squared (X²)-tests (A). The number of genes associated with differentially methylated regulatory sequences containing or not containing ERE was similar in the CMA3− sorted sperm fractions of infertile patients and healthy donors. Based on the X²-values, heat maps were constructed to visualize differences between observed and expected numbers of associated genes in the various comparisons (B). There were no consistent differences in the observed and expected numbers of genes associated to estrogenic or non-estrogenic DMR in the sperm fractions sorted based on CMA3− uptake of healthy donors and infertile patients