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Fig. 7 | Clinical Epigenetics

Fig. 7

From: THOR is a targetable epigenetic biomarker with clinical implications in breast cancer

Fig. 7

Histological analysis of TETg7_MO demethylation effect in MCF-7 xenotransplants, magnification 20x. 1.6 × 105 MCF-7 cells were subcutaneous injected into NOD/SCID mice, and tumor sizes were monitored over time using a caliper, being tumor volume calculated according to the formula, V = L x W x H /2 (V- volume, L- length, W- width, H- height). 88 days after MCF-7 cells injection, tumor tissue was isolated, formalin-fixed, and paraffin-embedded for histological analysis by hematoxylin and eosin staining. NT, MOCK and TET_MO xenotransplants correspond to densely cellular tumors, composed of polygonal cells with solid pattern in scant fibrous stroma. Cells have variably indistinct cell borders, scant amounts of eosinophilic cytoplasm, round to oval nuclei vesicular or with finely stippled chromatin; there is also marked anisocytosis and anisokaryosis, and mitotic figures (white arrowhead), on average 1–2 per one 40 × high-power field (HPF). In contrast, TETg7_MO xenotransplants are composed of polygonal cells arranged in haphazard islands separated by variably thick bands of fibrous connective tissue, and with a prominent basal lamina (black arrowhead). Cells show only moderate anisocytosis and anisokaryosis, with smaller nuclei, more abundant and clear cytoplasm, and mitotic figures are less frequent, averaging 1–2 per five 40 × HPF

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