An examination of mediation by DNA methylation on birthweight differences induced by assisted reproductive technologies

Table 2 Effects of DNA methylation on birthweight differences between fresh and naturally conceived newborns

	Total effect^{1, 2}	P value
Naturally conceived (reference) vs fresh embryo transfer	− 120 (− 179, − 61)	6.35E−05

CpG name	Indirect effect^{1, 2} in grams	P value¹	Direct effect^{1, 2} in grams	P value	Mediation proportion	CHR³	MAPINFO³	UCSC RefGene Name³
cg10372921	− 23 (− 34, − 13)	3.49E−05	− 98 (− 156, − 38)	1.22E−03	20.4%	15	74,218,733	LOXL1⁴
cg25423077	− 16 (− 27, − 8)	1.04E−03	− 104 (− 163, − 45)	5.85E−04	14.5%	18	59,221,601	CDH20⁵
cg02050426	− 15 (− 26, − 7)	1.41E−03	− 105 (− 164, − 46)	4.94E−04	13.5%	18	59,221,458	CDH20⁵
cg15138396	− 15 (− 27, − 6)	3.98E−03	− 105 (− 165, − 44)	5.86E−04	13.6%	2	26,624,450	DRC1⁶

	Combined indirect effect^{1, 2}	P value¹	Combined direct effect^{1, 2}	P value	Mediation proportion
The four CpGs combined	− 53 (− 71, − 37)	9.50E−10	− 67 (− 125, − 6)	2.86E−02	44.1%

¹Adjusted for maternal age, education, smoking status, pre-pregnancy BMI, parity, child’s sex, maternal intake of folic acid, and plate number. Birthweight difference is in grams
²95% confidence intervals in parenthesis
³Derived from the Illumina MethylationEPIC v1.0 B5 manifest file. The archaic names of the genes were updated based on the data from https://www.genenames.org/
⁴Located within 0–200 bases upstream of the transcription start site of the gene
⁵Located within the gene body
⁶Located within 200–1500 bases upstream of the transcription start site of the gene

ISSN: 1868-7083