Fig. 2

Differentially methylated CpGs between newborns conceived naturally and by fresh embryo transfer and birthweight-associated CpGs. a Manhattan plot displaying the 237 differentially methylated CpGs between newborns conceived naturally and those conceived by fresh embryo transfer. The red dotted line refers to the Bonferroni threshold (P = 0.05/770,564). Adjustment variables include maternal age, smoking status, pre-pregnancy BMI, parity, offspring sex, and plate number. b Quantile–quantile plot showing the birthweight-associated CpGs among the 237 fresh embryo transfer-associated CpGs. The yellow dots refer to the CpGs that were also associated with birthweight. Adjustment variables include those mentioned in (a) and maternal education and intake of folic acid. c Quantile–quantile plot showing the interactions of fresh embryo transfer and CpGs on birthweight. Adjustment variables were those mentioned in (b)