Fig. 3
From: Investigating pathological epigenetic aberrations by epi-proteomics
Schematic bottom-up workflow for MS-based analysis of histone PTMs and variants. Histones are enriched through specific protocols from different types of clinical samples, separated by SDS-PAGE and in-gel digested. Digested peptides are separated by liquid chromatography and acquired in the mass spectrometer. MS spectra are then used for peptide identification and quantification, and PTM assignment