Fig. 4

The HDAC inhibitor, SAHA, restores the expression of the PRELP gene through H2B acetylation at the promoter regions in the bladder cancer cells. A Genomic positions (Region 1 and Region 2) of the two PCR-amplified DNAs fragments are shown. B ChIP-qPCR was performed using various histone antibodies at the PRELP gene promoter region (Region 1) in RT4 cells treated with DMSO or 5 μM SAHA for 24 h. C Same as (B), except that region 2 was similarly analyzed. D The acetylation status of H2BK5 in the promoter region (Region 1 and Region 2) of PRELP gene in RT4 cells treated with two inhibitors, C646 (5 μM), SGC-CBP30 (5 μM), in the presence of SAHA (5 μM). Black; DMSO, Red; SAHA, Blue; SAHA with C646, Green; SAHA with SGC-CBP30. E H2BK5ac ChIP-qPCR in J82 cells. The ChIP enrichment (red bars) is normalized against the values obtained with the same antibodies in DMSO-treated control cells (black bars). Bars represent the mean ± SD of three technical replicates or mean ± standard error of the mean (SEM) of three biological replicates. Statistical analysis was performed using paired Student’s t test. *P < 0.05, **P < 0.01